ABSTRACT
Objective: Andrographis paniculata is a well-known medicinal plant in Southeast Asia, India and China. The plant contains andrographolide (AN), a very important phytochemical used in various health problems. However, AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity. Methods: The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid (soya phosphatidylcholine) in order to increase the oral absorption and antihyperglycemic activity in rats. Results: The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg /kg AN significantly protected the hyperglycemic condition of rats. The in vitro and in vivo experiments results proved that the nano- phytovesicular system of plant extracts containing AN produced better oral absorption, bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg. Conclusion: Hence, the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN.
ABSTRACT
The present work objective are to investigate indigenous plants used in wound healing in India, we hereby reported our findings related to wound healing activities of plant Solanum xanthocarpum Schrad and Wendl [Solanaceae] from some in vitro and in vivo model studies. The plant ethanolic extracts of the leaves of Solanum xanthocarpum was identified qualitative phytochemical constituents and tested wound healing activity. The important secondary metabolites alkaloid, glycosides, saponins, carbohydrates, tannins, phenolic compounds, protein, and fats were identified in extracts. Solanum xathocarpum leaves ethanolic extract showed wound healing activity significantly in the excision and incision wound model in rats on topical application. The animals were divided into five groups with six rats in each group. Topically applied 10% w/v of plant Solanum xanthocarpum leaves extracts in saline taking Silver Sulphadizine ointment as standard. The results showed that ethanolic extract of Solanum xathocarpum leaves on topical application was reduced the epithelization period from 25.30 +/- 0.23 to 19.75 +/- 0.28 days control and ethanol extract respectively along with a marked decrease in the scar area from 53.88 +/- 0.42 to 37.76 +/- 0.17 mm[2] control and ethanol extract respectively. Significant increase in tensile strength and hydroxyproline content of plant extract were also observed and compared to the control and silver sulphadiazine. The above result revealed that the ethanol extract has remarkable wound healing potency and appear to justify the traditional use of Solanum xanthocarpum in wound healing in India and offer a scientific support to the treatment of traditional healers